Radar degli Studi Clinici

Change in PhenoAge from Baseline to Week 12

Phenotypic Age (PhenoAge) is a composite biomarker-based estimate of biological age. The primary outcome is the change in PhenoAge from baseline to the end of the 12-week intervention period. PhenoAge will be calculated using the algorithm developed by Levine et al. (2018), based on a validated mortality risk model.

Baseline and Week 12

Change in Calcium Content of Bone

The calcium content of bone will be measured at baseline and at Week 12 to evaluate changes resulting from CaAKG supplementation. Bone calcium will be assessed using dual-energy X-ray absorptiometry (DXA) with mineral content analysis at specific skeletal sites. The primary metric is the change in bone calcium content (mg/cm2 or mg) over the 12-week intervention period.

Baseline and Week 12

Change in β-Galactosidase Activity

β-Galactosidase activity will be measured in serum collected at baseline and at Week 12.

Baseline and Week 12

Change in Age-related Gene Expression

e.g., SIRT1\~7、TERT/TERC、p21(CDKN1A)、p16INK4a(CDKN2A)、PDGFRB、PLOD1、MAP4K4、NFKBIA、GFAP、F2RL3、HERPUD1、PPPIR15A、PCBP1、TFAM、MMP7

Baseline and Week 12

Change in Grip strength

The change in Grip strength (kg) of 30 volunteers before and after the intervention was measured.

Baseline,12-week study

Change in body Weight

The change in body weight (kg) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in body mass index(BMl)

The change in Body Mass Index (BMl) kg/m2 of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in body fat mass

The change in body fat mass(kg) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in alkaline phosphatase (ALP)

The change in alkaline phosphatase (ALP) in U/L of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in body fat percentage

The change in body fat percentage (%) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in fat-free mass

The change in fat-free mass(kg) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in muscle mass

The change in muscle mass (kg) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in body water

The change in body water (kg) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in blood creatinine (CREA)

The change in blood creatinine (CREA) in mol/L of 30 volunteers before and after the intervention was measured.

Baseline and 12-week study.

Change in protein

The change in protein (kg) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in inorganic salts

The change in inorganic salts (kg) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in TotalT lymphocytes (CD3+)

The change in TotalT lymphocytes (CD3+) (cells/μl) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in helper T cells(cD4+)

The change in helper T cells (CD4+) (cells/μl) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in cytotoxicT cells(CD8+)

The change in cytotoxic T cells (CD8+)(cells/μL) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in Glycated haemoglobin (HbAlc)

The change in Glycated haemoglobin (HbA1c) (%) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in Fasting blood glucose

The change in Fasting blood glucose (mmol/L) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in Fasting insulin

The change in Fasting insulin (pmol/L) of 30 volunteers before and after the intervention was measured.

Baseline and Week 12

Change in Physical activity questionnaire Scores

International Physical Activity Questionnaire - Long Form (IPAQ-LF). At baseline and 12 weeks, measure changes in participants' Physical activity questionnaire scores. There is no fixed upper or lower limit to the total score, but scores at different activity intensities (walking, moderate intensity, high intensity) can be accumulated based on metabolic equivalents (MET-min/week). Higher score: Reflects the greater the total amount of physical activity an individual has engaged in in the past week.

Baseline and Week 12

Change in number of finger taps in one minute

The change in number of finger taps in one minute of 30 volunteers before and after the intervention was measured.

Baseline and Week 12