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メイヨー・クリニックA Study of Adipose Tissue in Adaptive Responses to Exercise
治験(臨床試験)の詳細は主に英語で提供されています。しかし、治験レーダーAIが支援できます！「治験を説明」をクリックして、選択した言語で試験情報を表示し、議論してください。
治験番号 NCT06053125 は 介入研究 臨床試験 で、健康、肥満 に関するものです。現在は 実施中／登録終了 で、2023年11月9日 から開始しています。56 名の参加者 の募集が計画されています。この治験は メイヨー・クリニック によって主催され、2026年10月1日 に完了予定です。ClinicalTrials.gov からの最新更新日は 2025年10月23日 です。
概要
The purpose of this research is to determine how exercise affects fat (adipose) tissue and how changes to adipose tissue that occur during and after exercise might improve health in aging and obesity.
詳細説明
Adipose tissue has important endocrine functions that influence metabolic health. Early evidence shows that adipose adapts to physiological stress, including exercise. The objective here is to determine how exercise-induced alterations in adipose tissue cellular composition and endocrine signaling may contribute to the beneficial adaptations to exercise in aging and obesity.
Immune cell populations and inflammatory signatures will be assessed in subcutaneous abdominal adipose tissue biopsies collected from obese and normal weight young and older adults before, immediately after, and 3 hours after a 30-min bout of cycling exercise at 70% of maximal oxygen consumption (VO2max). Mass spectrometry, Olink targeted inflammation assays, and RNA sequencing will be used for full proteomic and transcriptomic characterization of the adipose tissue secretome (the proteins and molecules secreted from the adipose tissue) and the cargo of extracellular vesicles isolated from plasma and media collected from cultured human adipose tissue explants generated from each time point.
Overall, the primary hypothesis of the proposed work is that a single bout of exercise triggers transient changes in adipose tissue paracrine/endocrine signals and immune cellular composition. The investigators propose that these responses contribute to the beneficial effects of exercise locally and in distal tissues and that the cumulative effects of acute changes in adipose tissue likely contribute to the positive alterations in adipose tissue associated with exercise training.
公式タイトル
The Role of Adipose Tissue in Adaptive Responses to Exercise
疾患／病気
健康肥満その他の研究識別子
- 23-002049
- K01DK134765 (米国NIHの助成金/契約)
NCT番号
開始日
2023-11-09
最終更新日
2025-10-23
終了予定日
2026-10
目標参加者数
56
試験の種類
介入研究
治験の相・段階
該当なし
状況
実施中／登録終了
キーワード
exercise
adipose
cytokines
adipose
cytokines
主目的
基礎研究
割付方法
非無作為化
介入モデル
並行割当
盲検化
なし（非盲検）
群（アーム）／介入
| 参加グループ/群 | 介入/治療法 |
|---|---|
実験的Older adults with obesity Participants with obesity as determined by the principal investigator and aged 65-85 years will have an adipose tissue biopsy taken from the abdomen before and after exercise. | 脂肪組織生検 Fat sample collected from abdomen before exercise, immediately after exercise, and 3 hours after exercise 運動 30 minutes of exercise on a cycle ergometer exercise machine |
実験的Older adults without obesity Participants with obesity as determined by the principal investigator and aged 65-85 years will have an adipose tissue biopsy taken from the abdomen before and after exercise. | 脂肪組織生検 Fat sample collected from abdomen before exercise, immediately after exercise, and 3 hours after exercise 運動 30 minutes of exercise on a cycle ergometer exercise machine |
実験的Young adults with obesity Participants with obesity as determined by the principal investigator and aged 18-35 years will have an adipose tissue biopsy taken from the abdomen before and after exercise. | 脂肪組織生検 Fat sample collected from abdomen before exercise, immediately after exercise, and 3 hours after exercise 運動 30 minutes of exercise on a cycle ergometer exercise machine |
実験的Young adults without obesity Participants with obesity as determined by the principal investigator and aged 18-35 years will have an adipose tissue biopsy taken from the abdomen before and after exercise. | 脂肪組織生検 Fat sample collected from abdomen before exercise, immediately after exercise, and 3 hours after exercise 運動 30 minutes of exercise on a cycle ergometer exercise machine |
主要評価項目
副次評価項目
| 評価指標 | 指標の説明 | 時間枠 |
|---|---|---|
Adipose lymphocyte populations | The numbers of lymphocytes in the adipose tissue will be assessed by flow cytometry. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
IL6 secreted from adipose tissue measured using the Olink Target Inflammation Panel (includes 96 inflammation-associated proteins) | The concentration of 96 IL6 will be measured using the targeted inflammation Olink proximity extension assay panel in media obtained from cultured adipose tissue explants. Adipose tissue samples will be cultured for 3 hours, and the culture media will be removed for the assessment of secreted inflammatory proteins. T | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Adipose tissue macrophage populations | Numbers of macrophages in the adipose tissue will be assessed by immunohistochemistry. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Adipose tissue gene expression of interleukin (IL)-6 measured by polymerase chain reaction (PCR). | Gene expression of the inflammatory cytokine IL-6 will be measured in adipose tissue samples using real time (RT)-PCR. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Adipose tissue gene expression of interleukin (IL)-8 measured by PCR. | Gene expression of the inflammatory cytokine IL-8 will be assessed in adipose tissue samples using real-time (RT)-PCR. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Adipose tissue gene expression of cluster of differentiation 68 (CD68) measured by PCR. | Gene expression of the macrophage marker CD68 be assessed in adipose tissue samples using real time (RT)-PCR. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Adipose tissue gene expression of cluster of differentiation 163 (CD163) measured by PCR. | Gene expression of the anti-inflammatory macrophage marker CD163 will be assessed in adipose tissue samples using RT-PCR. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Adipose tissue gene expression of cluster of differentiation 206 (CD206) measured by PCR. | Gene expression of the anti-inflammatory macrophage marker CD206 will be assessed in adipose tissue samples using RT-PCR. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Adipose tissue gene expression of adhesion G-protein coupled receptor E (ADGRE) measured by PCR. | Gene expression of the macrophage marker ADGRE will be assessed in adipose tissue samples using RT-PCR. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Adipose tissue gene expression of inducible nitric oxide synthase (INOS) measured by PCR. | Gene expression of the pro-inflammatory macrophage marker INOS will be assessed in adipose tissue samples using RT-PCR. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Adipose tissue gene expression of monocyte chemoattractant protein-1 (MCP1) measured by PCR. | Gene expression of the inflammatory cytokine MCP1 will be assessed in adipose tissue samples using RT-PCR. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Adipose tissue gene expression of tumor necrosis factor alpha (TNFa) measured by PCR. | Gene expression of the inflammatory cytokine TNFa will be assessed in adipose tissue samples using RT-PCR. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Adipose tissue gene expression of leptin (LEPD) measured by PCR. | Gene expression of the pro-inflammatory adipokine LEPD will be assessed in adipose tissue samples using RT-PCR. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Adipose tissue gene expression of adiponectin (ADIPOQ) measured by PCR. | Gene expression of the anti-inflammatory adipokine ADIPOQ will be assessed in adipose tissue samples using RT-PCR. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Inflammatory proteins found in adipose tissue measured using the Olink Target Inflammation Panel (includes 96 inflammation-associated proteins) | The concentrations of 96 different inflammatory proteins (including various cytokines and adipokines) will be measured in adipose tissue lysates using the targeted inflammation Olink proximity extension assay panel. This assay measures all 96 proteins in a single assay and all are reported as normalized protein expression values. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Protein cargo of adipose extracellular vesicles measured using mass spectrometry | Adipose tissue samples will be cultured for 3 hours, and the culture media will be removed. From this media, extracellular vesicles will be isolated. In these extracellular vesicles, mass spectrometry will be performed to examine the entire proteome (all proteins) contained within these extracellular vesicles. Using the large number of proteins identified, the investigators will examine changes in categories of protein types (e.g., inflammatory proteins, metabolic proteins, transcriptional proteins). | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
| 評価指標 | 指標の説明 | 時間枠 |
|---|---|---|
Adipocyte cell size | Adipocyte cell size will be determined using methylene blue staining and microscopy. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Plasma IL6 concentrations measured using the Olink Target Inflammation Panel | The concentration of IL6 will be measured in plasma samples using the targeted inflammation Olink proximity extension assay panel. This assay measures 96 proteins in a single assay and all are reported as normalized protein expression values. | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
Protein cargo of plasma extracellular vesicles measured using mass spectrometry extracellular vesicles | Extracellular vesicles will be isolated from plasma samples. In these extracellular vesicles, mass spectrometry will be performed to examine the entire proteome (all proteins) contained within these extracellular vesicles. Using the large number of proteins identified, the investigators will examine changes in categories of protein types (e.g., inflammatory proteins, metabolic proteins, transcriptional proteins). | Pre-exercise, immediately post-exercise, and 3 hours after exercise |
適格基準
対象年齢
成人, 高齢者
試験の最低年齢
18 Years
対象性別
全て
健康なボランティアを受け入れる
はい
- Adults between the ages of 18-35 OR 65-85.
- BMI 18.5 kg/m2 - 40 kg/m2
- Weight stable (≥ 3 months)
- Generally healthy
- Participation in ≥ 30 minutes of structured physical activity ≥ 2 days per week.
- Smoking/tobacco use.
- Alcohol/substance abuse.
- Pregnancy and breastfeeding.
- Anemia.
- Abnormal renal function.
- Blood clotting disorders.
- Coronary artery disease.
- Uncontrolled thyroid disease.
- Liver disease.
- Use of medications known to influence the main outcomes of the study.
- Orthopedic problems that may be aggravated by exercise.
- Chronic disease at the discretion of the investigators.
メイヨー・クリニック868 件のアクティブな治験を探索National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)責任者
Hawley E. Kunz, 研究責任者, Principal Investigator, Mayo Clinic
連絡先情報がありません。
1 1カ国の場所
Minnesota
Mayo Clinic, Rochester, Minnesota, 55905, United States